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Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope
Robin, Aelig; Koehler, Valentin; Jones, Alison; Ali, Afruja; Kelly, Paul P; O'Reilly, Elaine; Turner, Nicholas J; Flitsch, Sabine L
Beilstein Journal of Organic Chemistry. 2011;7:1494-1498.
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Abstract
A high-throughput screening protocol for evaluating chimeric, self-sufficient P450 biocatalysts and their mutants against a panel of substrates was developed, leading to the identification of a number of novel biooxidation activities.
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