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Visualization and characterisation of defined hair follicle compartments by Fourier transform infrared (FTIR) imaging without labelling.

Lau, Katherine; Hedegaard, Martin A B; Kloepper, Jennifer E; Paus, Ralf; Wood, Bayden R; Deckert, Volker

Journal of dermatological science. 2011;63(3):191-8.

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Abstract

BACKGROUND: To visualise and characterise skin architecture, the tissue usually has to be destroyed and labelled. OBJECTIVES: The use of Fourier transform infrared (FTIR) spectroscopy as a label-free, minimally sample destructive method to define hair follicular structure has been explored and demonstrated in this paper. METHODS: Human scalp skin cryosections were imaged using FTIR microscopy and the data was subsequently analysed with N-FINDR spectral unmixing algorithm. RESULTS: This resulted in an excellent distinction of known hair follicle tissue layers, which could be discerned based on their molecular structure. CONCLUSION: The development of a minimally sample-destructive, label-free spectroscopy based technique that can differentiate layers of cells in the dermal papilla and connective tissue sheath in the mesenchyme of the hair follicle paves the way forward to identifying spectral markers important in wound healing and stem cell therapies.

Bibliographic metadata

Type of resource:
Content type:
Publication type:
Published date:
Abbreviated journal title:
ISSN:
Place of publication:
Netherlands
Volume:
63
Issue:
3
Pagination:
191-8
Digital Object Identifier:
10.1016/j.jdermsci.2011.05.002
Pubmed Identifier:
21752602
Pii Identifier:
S0923-1811(11)00150-2
Access state:
Active

Institutional metadata

University researcher(s):

Record metadata

Manchester eScholar ID:
uk-ac-man-scw:169052
Created by:
Paus, Ralf
Created:
12th September, 2012, 13:36:24
Last modified by:
Paus, Ralf
Last modified:
12th September, 2012, 13:36:24

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