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Rat bone marrow mesenchymal stem cells express glial markers and stimulate nerve regeneration.
Tohill MP, Mantovani C, Wiberg M, Terenghi G
Neurosci Lett. 2004;362( 3):200-3
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Abstract
Bone marrow mesenchymal stem cells can trans-differentiate into neuronal phenotypes. We examined the differentiation of marrow stromal cells (MSCs) in culture and during nerve regeneration. MSCs from adult rats were exposed to glial growth factor (GGF) to stimulate glial differentiation. Subsequently differentiated MSCs were retrovirally labelled with green fluorescent protein and transplanted into 1 cm nerve conduits in the rat sciatic nerve. Fifteen days post-operatively the conduits were examined for axonal and Schwann cell regeneration and MSC integration. In vitro, MSCs exposed to GGF expressed S100 and glial fibrillary acidic protein. Following transplantation, MSCs maintained S100 expression and enhanced nerve regeneration, with significant Schwann cell regeneration compared to control (2.7 +/- 0.21 vs. 2.05 +/- .21 mm; P < 0.05). MSCs not exposed to GGF prior to transplantation expressed S100 in vivo indicating glial differentiation in response to local cytokines and growth factors. Copyright 2004 Elsevier Ireland Ltd.
Keyword(s)
Animals; Comparative Study; Gene Expression Regulation; Green Fluorescent Proteins; Rats; Rats, Sprague-Dawley; Research Support, Non-U.S. Gov't; cytology: Bone Marrow Cells; drug effects: Cell Differentiation; drug effects: Stromal Cells; metabolism: Glial Fibrillary Acidic Protein; metabolism: Luminescent Proteins; metabolism: Neuregulin-1; metabolism: Retroviridae; metabolism: S100 Proteins; methods: Bone Marrow Transplantation; methods: Cell Count; methods: Immunohistochemistry; physiology: Nerve Regeneration; transplantation: Sciatic Nerve
Bibliographic metadata
- Animals
- cytology: Bone Marrow Cells
- methods: Bone Marrow Transplantation
- methods: Cell Count
- drug effects: Cell Differentiation
- Comparative Study
- Gene Expression Regulation
- metabolism: Glial Fibrillary Acidic Protein
- Green Fluorescent Proteins
- methods: Immunohistochemistry
- metabolism: Luminescent Proteins
- physiology: Nerve Regeneration
- metabolism: Neuregulin-1
- Rats
- Rats, Sprague-Dawley
- Research Support, Non-U.S. Gov't
- metabolism: Retroviridae
- metabolism: S100 Proteins
- transplantation: Sciatic Nerve
- drug effects: Stromal Cells