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An asparagine oxygenase (AsnO) and a 3-hydroxyasparaginyl phosphotransferase (HasP) are involved in the biosynthesis of calcium-dependent lipopeptide antibiotics

J. Neary, A. Powell, L. Gordon, C. Milne, F. Flett, B. Wilkinson, C.P. Smith and J. Micklefield

Microbiology. 2007;153:768-776.

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Abstract

Nonribosomal peptides contain a wide range of unusual non-proteinogenic amino acid residues. As a result, these complex natural products are amongst the most structurally diverse secondary metabolites in nature, and possess a broad spectrum of biological activities. Beta-Hydroxylation of amino acid precursors or peptidyl residues and their subsequent processing by downstream tailoring enzymes are some of the most common themes in the biosynthetic diversification of these therapeutically important peptides. Identification and characterization of the biosynthetic intermediates and enzymes involved in these processes are thus pivotal in understanding nonribosomal peptide assembly and modification. To this end, the putative asparaginyl oxygenase- and 3-hydroxyasparaginyl phosphotransferase-encoding genes hasP and asnO were separately deleted from the calcium-dependent antibiotic (CDA) biosynthetic gene cluster of Streptomyces coelicolor. Whilst the parent strains produce a number of 3-hydroxyasparagine- and 3-phosphohydroxyasparagine-containing CDAs, the delta-hasP mutants produce exclusively non-phosphorylated CDAs. On the other hand, asnO mutants produce several new Asn-containing CDAs not present in the wild-type, which retain calcium-dependent antimicrobial activity. This confirms that AsnO and HasP are required for the beta-hydroxylation and phosphorylation of the Asn residue within CDA.

Bibliographic metadata

Type of resource:
Content type:
Publication type:
Publication form:
Published date:
Journal title:
ISSN:
Volume:
153
Start page:
768
End page:
776
Total:
9
Pagination:
768-776
Digital Object Identifier:
10.1099/mic.0.2006/002725-0
Access state:
Active

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Record metadata

Manchester eScholar ID:
uk-ac-man-scw:1a771
Created:
6th August, 2009, 08:59:02
Last modified by:
Micklefield, Jason
Last modified:
16th October, 2009, 12:04:41

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