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Relative quantification in proteomics: new approaches for biochemistry.

Unwin RD, Evans CL, Whetton A

Trends in Biochemical Sciences. 2006;31:473-84.

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Abstract

Recent developments in mass spectrometry and protein arrays provide opportunities to derive systematically proteomic information from small samples of cellular material. Relative quantification among samples can be achieved with either gel-based or gel-free approaches. Furthermore, the adaptation of specific techniques facilitates absolute quantification. Here, relative quantification in two-dimensional gel electrophoresis is contrasted with that in non-gel-based approaches such as isobaric tagging of peptides, pre-labelling of living cells with isotopomeric forms of essential amino acids and protein array platforms. In addition, novel flow-cytometry-based approaches are considered. These technologies can all be used to determine accurately the levels of proteins or biomarkers in a wide range of samples.

Bibliographic metadata

Type of resource:
Content type:
Publication type:
Publication form:
Published date:
Volume:
31
Start page:
473
End page:
84
Pagination:
473-84
Digital Object Identifier:
10.1016/j.tibs.2006.06.003
Access state:
Active

Institutional metadata

University researcher(s):

Record metadata

Manchester eScholar ID:
uk-ac-man-scw:1d13562
Created:
30th August, 2009, 12:50:01
Last modified:
1st February, 2015, 19:11:44

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