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cGMP-dependent protein kinase activates Ca-activated K channels in cerebral artery smooth muscle cells.
Robertson B, Schubert R, Hescheler J, Nelson MT
American Journal of Physiology-Cell Physiology. 1993;265( 1 Pt 1):C299-303.
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Abstract
Guanosine-3',5'-cyclic monophosphate (cGMP)-dependent protein kinase (cGMP-PK) plays a central role in the mediation of the vasodilator response to nitric oxide (NO) and other nitrovasodilators. It is unclear whether cGMP-PK affects calcium-activated potassium channels (KCa channels) or any other type of ion channel in smooth muscle. We provide here the first direct evidence that cGMP-PK can activate KCa channels in arterial smooth muscle cells. We demonstrate that NO and a membrane-permeable analogue of cGMP can activate KCa channels in on-cell patches approximately twofold. Furthermore, cGMP-PK, in the presence of ATP and cGMP added directly to the intracellular surface of inside-out patches, increases channel activity by approximately eightfold. These results suggest that cGMP-PK-mediated activation of KCa channels may contribute to the actions of NO and other nitrovasodilators.
Keyword(s)
Animals; Cell Membrane Permeability; Rabbits; cytology: Cerebral Arteries; cytology: Muscle, Smooth, Vascular; drug effects: Potassium Channels; pharmacology: Calcium; pharmacology: Nitric Oxide; pharmacology: Protein Kinases