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cGMP-dependent protein kinase activates Ca-activated K channels in cerebral artery smooth muscle cells.

Robertson B, Schubert R, Hescheler J, Nelson MT

American Journal of Physiology-Cell Physiology. 1993;265( 1 Pt 1):C299-303.

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Abstract

Guanosine-3',5'-cyclic monophosphate (cGMP)-dependent protein kinase (cGMP-PK) plays a central role in the mediation of the vasodilator response to nitric oxide (NO) and other nitrovasodilators. It is unclear whether cGMP-PK affects calcium-activated potassium channels (KCa channels) or any other type of ion channel in smooth muscle. We provide here the first direct evidence that cGMP-PK can activate KCa channels in arterial smooth muscle cells. We demonstrate that NO and a membrane-permeable analogue of cGMP can activate KCa channels in on-cell patches approximately twofold. Furthermore, cGMP-PK, in the presence of ATP and cGMP added directly to the intracellular surface of inside-out patches, increases channel activity by approximately eightfold. These results suggest that cGMP-PK-mediated activation of KCa channels may contribute to the actions of NO and other nitrovasodilators.

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Place of publication:
UNITED STATES
Volume:
265( 1 Pt 1)
Start page:
C299
End page:
303
Pagination:
C299-303
Access state:
Active

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Record metadata

Manchester eScholar ID:
uk-ac-man-scw:1d16448
Created:
30th August, 2009, 13:57:46
Last modified:
3rd March, 2010, 17:09:56

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