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Developmental Fate Determination and Marker Discovery in Hematopoietic Stem Cell Biology Using Proteomic Fingerprinting

Spooncer, E, Brouard, N, Nilsson, S, Williams, B, Liu, M, Unwin, R, Unwin, RD, Blinco, D, Jaworska, E, Simmons, P, Whetton, A.

Molecular & Cellular Proteomics. 2008;7(3):573-581.

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Abstract

In hematopoiesis, co-expression of Sca-1 and c-Kit defines cells (LS+K) with long-term reconstituting potential. In contrast, poorly characterised LS-K cells fail to reconstitute lethally-irradiated recipients. Relative quantification mass spectrometry and transcriptional profiling were employed to characterise LS+K and LS-K cells. This approach yielded data on >1200 proteins. Only 32% of protein changes correlated to mRNA modulation demonstrating post-translational protein regulation in early hematopoietic development. LS+K cells had lower expression of protein synthesis proteins but did express proteins associated with mature cell function. Major increases in erythroid development proteins were observed in LS-K cells, based on this assessment of erythroid potential we showed them to be principally erythroid progenitors demonstrating effective use of discovery proteomics for definition of primitive cells.

Bibliographic metadata

Type of resource:
Content type:
Publication type:
Publication form:
Published date:
Abbreviated journal title:
MCP
ISSN:
Volume:
7
Issue:
3
Start page:
573
End page:
581
Total:
9
Digital Object Identifier:
10.1074/mcp.M700292-MCP200
Access state:
Active

Institutional metadata

University researcher(s):

Record metadata

Manchester eScholar ID:
uk-ac-man-scw:1d16931
Created:
30th August, 2009, 14:08:52
Last modified by:
Bentley, Hazel
Last modified:
1st February, 2015, 19:17:33

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