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Developmental Fate Determination and Marker Discovery in Hematopoietic Stem Cell Biology Using Proteomic Fingerprinting
Spooncer, E, Brouard, N, Nilsson, S, Williams, B, Liu, M, Unwin, R, Unwin, RD, Blinco, D, Jaworska, E, Simmons, P, Whetton, A.
Molecular & Cellular Proteomics. 2008;7(3):573-581.
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Abstract
In hematopoiesis, co-expression of Sca-1 and c-Kit defines cells (LS+K) with long-term reconstituting potential. In contrast, poorly characterised LS-K cells fail to reconstitute lethally-irradiated recipients. Relative quantification mass spectrometry and transcriptional profiling were employed to characterise LS+K and LS-K cells. This approach yielded data on >1200 proteins. Only 32% of protein changes correlated to mRNA modulation demonstrating post-translational protein regulation in early hematopoietic development. LS+K cells had lower expression of protein synthesis proteins but did express proteins associated with mature cell function. Major increases in erythroid development proteins were observed in LS-K cells, based on this assessment of erythroid potential we showed them to be principally erythroid progenitors demonstrating effective use of discovery proteomics for definition of primitive cells.