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- DOI: 10.1128/MCB.02178-07
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How a cytokine is chaperoned through the secretory pathway by complexing with its own receptor: lessons from interleukin-15 (IL-15)/IL-15 receptor alpha.
Duitman E, Orinska Z, Bulanova E, Paus RR, Bulfone-Paus S
Mol Cell Biol. 2008;28( 15):4851-61.
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Full-text held externally
- DOI: 10.1128/MCB.02178-07
Abstract
While it is well appreciated that receptors for secreted cytokines transmit ligand-induced signals, little is known about additional roles for cytokine receptor components in the control of ligand transport and secretion. Here, we show that interleukin-15 (IL-15) translocation into the endoplasmic reticulum occurs independently of the presence of IL-15 receptor alpha (IL-15R alpha). Subsequently, however, IL-15 is transported through the Golgi apparatus only in association with IL-15R alpha and then is secreted. This intracellular IL-15/IL-15R alpha complex already is formed in the endoplasmic reticulum and, thus, enables the further trafficking of complexed IL-15 through the secretory pathway. Just transfecting IL-15R alpha in cells, which transcribe but normally do not secrete IL-15, suffices to induce IL-15 secretion. Thus, we provide the first evidence of how a cytokine is chaperoned through the secretory pathway by complexing with its own high-affinity receptor and show that IL-15/IL-15R alpha offers an excellent model system for the further exploration of this novel mechanism for the control of cytokine secretion.
Keyword(s)
Animals; Cell Line; Humans; Models, Biological; drug effects: Cell Membrane; drug effects: Endoplasmic Reticulum; drug effects: Intracellular Space; drug effects: Macrophages; drug effects: Monocytes; drug effects: Protein Transport; metabolism: Endonucleases; metabolism: Interleukin-15 Receptor alpha Subunit; metabolism: Molecular Chaperones; metabolism: Peptide Hydrolases; pharmacology: Dipeptides; pharmacology: Interferon-gamma; secretion: Interleukin-15