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IL-1beta induced changes in hypothalamic IL-1R1 and IL-1R2 mRNA expression in the rat.
Parker L, Rushforth D, Rothwell NJ, Luheshi G
Brain Res Mol Brain Res. 2000;79( 1-2):156-8.
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Abstract
Interleukin-1 receptor (IL-1R1 and IL-1R2) mRNA expression was detected within the rat hypothalamus, a primary site of IL-1 action, using RT-PCR. Levels of expression were unchanged by cardiac saline-perfusion. However, intracerebroventricular (i.c.v.) administration of IL-1beta caused changes in receptor mRNA expression in non-perfused animals that were profoundly different to those observed in their saline-perfused counterparts. This study demonstrates the importance of perfusing tissue to remove blood cells when determining changes in IL-1 receptor mRNA expression.
Keyword(s)
Animals; Injections, Intraventricular; Male; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; Support, Non-U.S. Gov't; administration & dosage: Interleukin-1; drug effects: Cerebral Ventricles; drug effects: Hypothalamus; drug effects: Transcription, Genetic; drug effects: Up-Regulation; genetics: Receptors, Interleukin-1; metabolism: RNA, Messenger