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Chronic myeloid leukaemia: an investigation into the role of Bcr-Abl-induced abnormalities in glucose transport regulation.
Barnes K, McIntosh E, Whetton A, Daley G, Bentley J, Baldwin S
Oncogene. 2005;24( 20):3257-67.
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Abstract
In chronic myeloid leukaemia (CML) expression of the chimeric tyrosine kinase, Bcr-Abl, promotes the inappropriate survival of haemopoietic stem cells by a nonautocrine mechanism in the absence of IL-3. Stimulation of glucose uptake appears to play an important role in the suppression of apoptosis by this cytokine in normal haemopoietic cells. To investigate whether the cell survival mechanisms mediated by the oncoprotein and cytokine showed any similarities, we employed a haemopoietic cell line, TonB210, engineered for inducible expression of Bcr-Abl. Tyrosine kinase expression in cytokine-deprived cells was found to mimic the effect of IL-3 in maintaining a higher V(max) for hexose uptake. In both IL-3- treated cells and those expressing Bcr-Abl, high rates of hexose uptake were associated with the retention at the cell surface of approximately 80% of the total cellular content of the GLUT1 glucose transporter. In contrast, treatment of Bcr-Abl-expressing cells for 6 h with the Bcr-Abl kinase inhibitor Glivec (10 muM), in the absence of IL-3, led to internalization of approximately 90% of the cell-surface transporters and drastically decreased (4.4+/-0.9 (mean+/-s.e.m., 4)-fold) the V(max) for hexose uptake, without significant effect on the K(m) for this process or on the total cellular transporter content. These effects were not the result of any significant loss in cell viability, and preceded the onset of apoptosis caused by inhibition of Bcr-Abl. Both IL-3 treatment and expression of Bcr-Abl led to enhanced phosphorylation of Akt (protein kinase B). The stimulation of transport by IL-3 and Bcr-Abl in TonB210 cells was inhibitable by phosphatidylinositol 3-kinase inhibitors, indicating the involvement of this kinase in the signal transduction pathway. These findings suggest that inhibition of glucose transport plays an important role in the therapeutic action of Glivec, and that the signal transduction pathways involved in transport stimulation by Bcr-Abl may offer novel therapeutic targets for CML.
Keyword(s)
Apoptosis; Biological Transport; Blotting, Western; Cell Survival; Dose-Response Relationship, Drug; Flow Cytometry; Humans; Immunohistochemistry; Kinetics; Phosphorylation; Research Support, Non-U.S. Gov't; Temperature; Time Factors; metabolism: 1-Phosphatidylinositol 3-Kinase; metabolism: Cytokines; metabolism: Deoxyglucose; metabolism: Fusion Proteins, bcr-abl; metabolism: Glucose; metabolism: Hematopoietic Stem Cells; metabolism: Interleukin-3; metabolism: Leukemia, Myeloid, Chronic; metabolism: Monosaccharide Transport Proteins; metabolism: Protein-Serine-Threonine Kinases; metabolism: Protein-Tyrosine Kinase; metabolism: Proto-Oncogene Proteins; pharmacology: Annexin A5; pharmacology: Doxycycline; pharmacology: Enzyme Inhibitors; pharmacology: Piperazines; pharmacology: Pyrimidines