Related resources
Search for item elsewhere
University researcher(s)
Academic department(s)
Identification of a truncated IL-18R beta mRNA: a putative regulator of IL-18 expressed in rat brain.
Andre R, Wheeler R, Collins P, Luheshi G, Pickering-Brown S, Kimber I, Rothwell NJ, Pinteaux E
J Neuroimmunol. 2003;145( 1-2):40-5.
Access to files
Full-text and supplementary files are not available from Manchester eScholar. Use our list of Related resources to find this item elsewhere. Alternatively, request a copy from the Library's Document supply service.
Abstract
Interleukin (IL)-18, a member of the IL-1 family, is a key mediator of peripheral inflammation and host defense responses, and has been implicated in inflammatory and neurodegenerative diseases in the brain. IL-18 acts via a receptor complex that closely resembles that of IL-1, consisting of a ligand binding protein, IL-18Ralpha, and an accessory protein, IL-18Rbeta. Here, we describe the presence of a splice variant of IL-18Rbeta that is predicted to encode a truncated soluble protein, consisting of only the first immunoglobulin-like domain of IL-18Rbeta (EMBL/Genbank accession number AJ550893). Both forms of IL-18Rbeta were expressed in rat cortex, striatum, hypothalamus, hippocampus, and also liver, and were detected in pure cultures of microglia, astrocytes and neurons. This novel splice variant is up-regulated rapidly in microglial cells by bacterial lipopolyssacharide (LPS). We propose that this putative truncated form of IL-18Rbeta is analogous to the soluble form of IL-1R accessory protein, and could act as an important regulator of IL-18 actions.
Keyword(s)
Amino Acid Sequence; Animals; Base Sequence; Cells, Cultured; Male; Mice; Molecular Sequence Data; Rats; Rats, Sprague-Dawley; Sequence Deletion; Solubility; Support, Non-U.S. Gov't; biosynthesis: Interleukin-18; biosynthesis: RNA, Messenger; cytology: Brain; genetics: Receptors, Interleukin; immunology: Alternative Splicing; immunology: Astrocytes; immunology: Microglia