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Involvement of corticotrophin releasing factor (CRF) in the thermogenic and anorexic actions of serotonin (5-HT) and related compounds.
Le Feuvre R, Aisenthal L, Rothwell NJ
Brain Res. 1991;555( 2):245-50.
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Abstract
The present study investigated the involvement of corticotrophin-releasing factor (CRF) in the thermogenic and anorexic actions of serotonin (5-HT) in the rat. Serotonergic compounds and CRF antibody were injected directly into the third ventricle of conscious, male Sprague-Dawley rats. Thermogenesis was measured as changes in whole body oxygen consumption by indirect calorimetry. Central injections of 5-HT (0.5-50 micrograms, i.c.v.) significantly increased resting oxygen consumption (VO2; maximum 12.5% increase), without obvious effects on behaviour. Similar increases in VO2 (12-17%) were observed following central injections of the 5-HT precursors, tryptophan (14 micrograms, i.c.v.) or 5-hydroxytryptophan (5-HTP, 20 micrograms, i.c.v.), and peripheral (10 mg/kg, i.p.) or central (30 micrograms, i.c.v.) injections of the 5-HT reuptake inhibitor, DL-fenfluramine. Administration of a polyclonal CRF antibody (3 microliters, i.c.v.) 10 min prior to serotonergic compounds, significantly reduced (77-106%) the increases in VO2 observed in response to central injections of 5-HTP (20 micrograms), 5-HT (50 micrograms) or peripheral injections of fenfluramine, but not those observed in response to either 30 micrograms fenfluramine (i.c.v.) or 20 micrograms 5-HT. Voluntary food intake was measured for 6 h in rats following 16 h starvation. Six-hour food intake was significantly reduced (30-60%) in rats given central injections of 5-HT or 5-HTP, and central or peripheral injections of fenfluramine.(ABSTRACT TRUNCATED AT 250 WORDS)
Keyword(s)
Animals; Dose-Response Relationship, Drug; In Vitro; Injections, Intraventricular; Male; Rats; Rats, Inbred Strains; Support, Non-U.S. Gov't; antagonists & inhibitors: Corticotropin-Releasing Hormone; drug effects: Body Temperature Regulation; drug effects: Feeding Behavior; drug effects: Oxygen Consumption; pharmacology: 5-Hydroxytryptophan; pharmacology: Fenfluramine; pharmacology: Serotonin; pharmacology: Tryptophan