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Cloning of rat brain interleukin-18 cDNA.

Culhane A, Hall M, Rothwell NJ, Luheshi G

Mol Psychiatry. 1998;3( 4):362-6.

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Abstract

The interleukin-1 (IL-1) family comprises IL-1 alpha and IL-1 beta and an endogenous IL-1 receptor antagonist (IL-1ra). IL-1 has diverse actions in the brain and has been implicated in both acute and chronic neurodegeneration. However, neither IL-1 alpha nor IL-1 beta are neurotoxic per se in vivo, so other IL-1 related ligands may be important in neurodegeneration. The cytokine interleukin-18 (also called interferon gamma inducing factor, IGIF) was first isolated from the liver of mice during toxic shock. It was later proposed as a member of the IL-1 family, based on protein sequence homology with IL-1 beta and IL-1ra, and has tentatively been called IL-1 gamma. We cloned IL-18 from adult rat brain and demonstrated, by RT-PCR, that it is expressed constitutively in cerebellum, hippocampus, hypothalamus, cortex and striatum. Rat brain IL-18 shows close homology to mouse and human IL-18, and to the recently published sequence from the rat adrenal gland. Mouse pro-IL-18 and pro-IL-1 beta are processed by caspase-1. We demonstrate that caspase-1 also cleaves rat IL-18 in vitro and that the caspase inhibitor, zVAD-DCB inhibits this cleavage.

Bibliographic metadata

Type of resource:
Content type:
Publication type:
Published date:
Journal title:
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Place of publication:
ENGLAND
Volume:
3( 4)
Start page:
362
End page:
6
Pagination:
362-6
Access state:
Active

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Academic department(s):

Record metadata

Manchester eScholar ID:
uk-ac-man-scw:1d29190
Created:
2nd September, 2009, 11:46:53
Last modified:
29th March, 2011, 13:09:07

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