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Array-based diagnostic gene-expression score for atopy and asthma.
Brutsche M, Joos L, Carlen Brutsche I, Bissinger R, Tamm M, Custovic A, Woodcock AA
J Allergy Clin Immunol. 2002;109( 2):271-3.
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Abstract
Whether gene expression is useful in discriminating different atopic phenotypes is unclear.The aim of the study was to evaluate a gene-expression score for thediagnosis of atopy and asthma and to assess disease activity as a guidefor therapeutic decisions. Purified mRNA from PBMCs of 18 atopic asthmaticsubjects, 8 atopic nonasthmatic subjects, and 14 healthy control subjectswas hybridized to cDNA membranes. A composite atopy gene expression (CAGE)score was determined by using 10 genes dysregulated in atopic individualsaccording to a specific algorithm. The CAGE score was better than totalIgE in differentiating atopic from nonatopic subjects (sensitivity, 96%;specificity, 92%). Correlation between the CAGE score and total IgE (P<.001) was found, and there was a trend for correlation with asthmaseverity (P =.051). The CAGE score was able to quantify phenotype-specificalteration in gene expression of atopic individuals. The CAGE score mightbe used as a diagnostic tool or to monitor the effects and side effects oftherapy.
Keyword(s)
Adolescent; Adult; Aged; Algorithms; Female; Gene Expression Profiling; Humans; Male; Middle Aged; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; ROC Curve; Sensitivity and Specificity; Severity of Illness Index; diagnosis: Asthma; diagnosis: Hypersensitivity, Immediate; diagnosis: Respiratory Hypersensitivity; genetics: RNA, Messenger; metabolism: Leukocytes, Mononuclear