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Annexin VIII is differentially expressed by chondrocytes in the mammalian growth plate during endochondral ossification and in osteoarthritic cartilage.

White A, Watson REB, Newman WG, Freemont AJ, Wallis GA

J Bone Miner Res. 2002;17( 10)(10).

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Abstract

Endochondral ossification is the developmental process that leads to the formation and coordinated longitudinal growth of the majority of the vertebrate skeleton. Central to this process is chondrocyte differentiation occurring in the growth plate that lies at the junction between the epiphyseal cartilage and the bone. To identify novel factors involved in this differentiation process, suppression subtractive hybridization was performed to amplify preferentially cDNAs uniquely expressed in fetal bovine growth plate chondrocytes as opposed to epiphyseal chondrocytes. The subtracted product was used to screen a fetal bovine chondrocyte cDNA library. One of the cDNA clones identified encoded the bovine orthologue of annexin VIII, a protein not previously described in the growth plate. Northern and Western blotting confirmed that annexin VIII was expressed by growth plate chondrocytes and not by epiphyseal chondrocytes. Immunohistochemistry of the fetal bovine growth plate identified a gradient of increasing annexin VIII protein from the proliferative to the hypertrophic zone. Immunofluorescence localized annexin VIII largely to the chondrocyte cell membrane. In a preliminary study, we examined the distribution of annexin VIII in normal and osteoarthritic (OA) articular cartilage. In OA cartilage, the protein was located in a subset of mid- to deep zone chondrocytes and in the matrix surrounding these cells; no annexin VIII was detected in normal articular cartilage. Thus annexin VIII is a marker for chondrocyte differentiation during normal endochondral ossification and may act as a marker for cells undergoing inappropriate differentiation in OA.

Bibliographic metadata

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Publication type:
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Published date:
Journal title:
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Place of publication:
United States
Volume:
17( 10)
Issue:
10
Digital Object Identifier:
10.1359/jbmr.2002.17.10.1851
General notes:
  • TY - JOURUI - 22256545 DA - 20021008 IS - 0884-0431 LA - eng PT - Journal Article RN - 0 (Annexins) RN - 0 (DNA, Complementary) RN - 0 (Membrane Proteins) SB - IMRP - NOT IN FILE
Access state:
Active

Record metadata

Manchester eScholar ID:
uk-ac-man-scw:1d9639
Created:
29th August, 2009, 15:06:26
Last modified by:
Ingram, Mary
Last modified:
9th July, 2015, 12:19:37

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