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Expression of microRNA-184 in keratinocytes represses argonaute 2.

Roberts, Julian C; Warren, Richard B; Griffiths, Christopher E M; Ross, Kehinde

Journal of cellular physiology. 2013;228(12):2314-23.

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Abstract

Interleukin-22 (IL-22) is a proinflammatory cytokine that has been associated with the pathogenesis of inflammatory skin disorders. However, the impact of IL-22 on microRNA (miRNA) expression in epidermal keratinocytes is unknown. Here we show that IL-22 induces miR-184 in reconstituted human epidermis (RHE) and in the HaCaT keratinocyte cell line. Exposure to IL-22 increased miR-184 expression 8- and 15-fold in RHE and HaCaT cells, respectively. Oncostatin M, an unrelated proinflammatory cytokine, also raised miR-184 expression in RHE and HaCaT keratinocytes. Pharmacologic and genetic inhibition demonstrated that cytokine-induced expression of miR-184 was mediated by signal transducer and activation of transcription 3 (STAT3). Argonaute 2 (AGO2), a member of the RNA-induced silencing complex (RISC), is a predicted miR-184 target. Using protein, messenger RNA and reporter analyses, we found that miR-184 regulates the expression of AGO2. We conclude that cytokine-induced miR-184 attenuates AGO2 expression in keratinocytes.

Bibliographic metadata

Type of resource:
Content type:
Publication type:
Published date:
Abbreviated journal title:
ISSN:
Place of publication:
United States
Volume:
228
Issue:
12
Pagination:
2314-23
Digital Object Identifier:
10.1002/jcp.24401
Pubmed Identifier:
23696368
Access state:
Active

Institutional metadata

University researcher(s):

Record metadata

Manchester eScholar ID:
uk-ac-man-scw:237725
Created by:
Sewell, Carol
Created:
23rd October, 2014, 11:00:18
Last modified by:
Sewell, Carol
Last modified:
23rd October, 2014, 11:00:18

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