In April 2016 Manchester eScholar was replaced by the University of Manchester’s new Research Information Management System, Pure. In the autumn the University’s research outputs will be available to search and browse via a new Research Portal. Until then the University’s full publication record can be accessed via a temporary portal and the old eScholar content is available to search and browse via this archive.

Related resources

University researcher(s)

    Academic department(s)

    An RNA-Seq approach to investigate light-controlled gene expression in Neurospora crassa with emphasis on red light-mediated responses

    Lia, Andrei-Stefan

    [Thesis]. Manchester, UK: The University of Manchester; 2015.

    Access to files

    Abstract

    ABSTRACTThe University of ManchesterAndrei-Stefan LiaMaster of PhilosophyAn RNA-Seq approach to investigate light-controlled gene expression in Neurospora crassa with emphasis on red light-mediated responses14th of October 2014With increasing numbers of genomes being sequenced, many phytochrome homologues have been identified across cyanobacteria, eubacteria and filamentous fungi. In recent years, two phytochrome homologues were identified in the filamentous fungus Neurospora crassa. Although functional and phenotypic analyses have been successful in other fungal species, the functional significance of Neurospora phytochromes remains unknown.In this body of work, we aim to investigate light-regulated gene expression via an RNA-Seq approach. Bioinformatic analysis of RNA-Seq data has not only shown that there is an independent red light response, but that this response is also controlled by the two phytochrome genes. Our analysis also confirms previously established light-regulated genes. We establish differentially expressed gene sets, from which we extracted cis-regulatory elements.Finally, we also show that phytochromes are mediators of a thermal acclimation response.

    Keyword(s)

    RNA-Seq; phytochrome

    Bibliographic metadata

    Type of resource:
    Content type:
    Form of thesis:
    Type of submission:
    Degree type:
    Master of Philosophy
    Degree programme:
    MPhil Genetics
    Publication date:
    Location:
    Manchester, UK
    Total pages:
    62
    Abstract:
    ABSTRACTThe University of ManchesterAndrei-Stefan LiaMaster of PhilosophyAn RNA-Seq approach to investigate light-controlled gene expression in Neurospora crassa with emphasis on red light-mediated responses14th of October 2014With increasing numbers of genomes being sequenced, many phytochrome homologues have been identified across cyanobacteria, eubacteria and filamentous fungi. In recent years, two phytochrome homologues were identified in the filamentous fungus Neurospora crassa. Although functional and phenotypic analyses have been successful in other fungal species, the functional significance of Neurospora phytochromes remains unknown.In this body of work, we aim to investigate light-regulated gene expression via an RNA-Seq approach. Bioinformatic analysis of RNA-Seq data has not only shown that there is an independent red light response, but that this response is also controlled by the two phytochrome genes. Our analysis also confirms previously established light-regulated genes. We establish differentially expressed gene sets, from which we extracted cis-regulatory elements.Finally, we also show that phytochromes are mediators of a thermal acclimation response.
    Keyword(s):
    Thesis main supervisor(s):
    Thesis advisor(s):
    Language:
    en

    Institutional metadata

    University researcher(s):
    Academic department(s):

    Record metadata

    Manchester eScholar ID:
    uk-ac-man-scw:259316
    Created by:
    Lia, Andrei-Stefan
    Created:
    12th February, 2015, 13:15:18
    Last modified by:
    Lia, Andrei-Stefan
    Last modified:
    13th September, 2018, 13:51:48

    Can we help?

    The library chat service will be available from 11am-3pm Monday to Friday (excluding Bank Holidays). You can also email your enquiry to us.