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Dynamics of Wolbachia pipientis gene expression across the Drosophila melanogaster life cycle.
Gutzwiller, F., Carmo, C. R., Miller, D. E., Rice, D. W., Newton, I. L., Hawley, R.S. Teixeira, L., & Bergman, C. M.
G3: Genes, Genomes, Genetics (Bethesda)..
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Accepted
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- NERC -
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Yes
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DNA-seq fastq reads for BDSC ISO1 were submitted to ENA as experiment ERX645969. Table
S1 contains a summary of the number of mapped RNA-seq reads for D. melanogaster and
Wolbachia, number of expressed genes (defined as genes with non-zero TPM or genes
with >2 mapped reads per gene), mean TPM for the sample (same for all samples, inverse
of gene number time one million), standard deviation of TPM for the sample for each
total RNA sample in SRP001696. Table S2 contains gene IDs, coordinates, number of
reads (from read 2 of paired-end data) mapping to the sense strand in each sample
( r1 = replicate 1, r2 = replicate 2), estimated TPM for each sample, number of runs
found in cluster 2, cluster assignment, adjusted p-value in life-cycle GLM, log2 fold-change
in life-cycle GLM vs embryo 0-2 hours, maximum fold change between any two stages
in life-cycle GLM, adjusted p-values and log2 fold change for pairwise exact tests
between male and female samples at 1, 5 and 30 days, gene name, annotated gene product,
effective number of codons, GC content, number of homologs in wMel, wRi, wPip-Pel,
and wBm genomes. Table S3 contains RT-qPCR GLM analysis results for ISO1 and DrosDel
w1118. P-values reported in Table S3 are not adjusted for multiple testing, and thus
-levels for significance were set at 0.001. Table S4 contains sequences of PCR primers
used for RT-qPCR experiments.
Attached files Open Access licence:
Creative Commons Attribution (CC BY)
Attached files embargo period:
Immediate release
Attached files release date:
12th November, 2015
Access state:
Active
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Manchester eScholar ID:
uk-ac-man-scw:277884
Created by:
Bergman, Casey
Created:
12th November, 2015, 20:00:08
Last modified by:
Bergman, Casey
Last modified:
6th March, 2016, 19:34:44