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Preparation of large numbers of plasmid DNA samples in microtiter plates by the alkaline lysis method.

Gibson, T J; Sulston, J E

Gene analysis techniques. 1987;4(3):41-4.

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Abstract

A protocol is described for the growth and preparation of plasmid DNAs from small culture volumes (250 microliters) and utilizing standard 96-well plates. Several hundred plasmids can be prepared simultaneously, yielding sufficient DNA for subsequent analysis by restriction digestion and gel electrophoresis. This protocol may be useful for rapid screening of clones arising in recombinant DNA work such as site-directed mutagenesis, oligonucleotide cassette cloning, deletion analysis, etc. The technique was initially developed to meet our requirement to provide large numbers of cosmid DNAs for restriction enzyme fingerprint analyses in genome mapping projects.

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Content type:
Published date:
Language:
eng
Journal title:
Abbreviated journal title:
ISSN:
Place of publication:
UNITED STATES
Volume:
4
Issue:
3
Pagination:
41-4
Pubmed Identifier:
3333759
Access state:
Active

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University researcher(s):
Academic department(s):

Record metadata

Manchester eScholar ID:
uk-ac-man-scw:67574
Created by:
Green, Linda
Created:
22nd October, 2009, 09:11:50
Last modified by:
Green, Linda
Last modified:
12th November, 2012, 20:11:48

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