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Lorist2, a cosmid with transcriptional terminators insulating vector genes from interference by promoters within the insert: effect on DNA yield and cloned insert frequency.

Gibson, T J; Coulson, A R; Sulston, J E; Little, P F

Gene. 1987;53(2-3):275-81.

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Abstract

Transcription terminators have been included in a phage-lambda-replicon-based cosmid vector, Lorist2, to insulate vector genes against transcriptional interference from cloned insert DNA. DNA yields of recombinant clones containing Escherichia coli genomic DNA inserts are more even for Lorist2 than with its progenitor LoristB. However, the terminators provide only a partial reduction in the over-representation of r X DNA-containing clones generally observed in cosmid libraries of Caenorhabditis elegans DNA, suggesting that causes other than transcriptional readthrough into the vector contribute to this problem.

Bibliographic metadata

Content type:
Published date:
Language:
eng
Journal title:
Abbreviated journal title:
ISSN:
Place of publication:
NETHERLANDS
Volume:
53
Issue:
2-3
Pagination:
275-81
Pubmed Identifier:
3301536
Access state:
Active

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University researcher(s):
Academic department(s):

Record metadata

Manchester eScholar ID:
uk-ac-man-scw:67576
Created by:
Green, Linda
Created:
22nd October, 2009, 09:12:04
Last modified by:
Green, Linda
Last modified:
12th November, 2012, 20:12:39

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