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Site-selective immobilisation of functional enzymes on to polystyrene nanoparticles.

Wong, Lu Shin; Okrasa, Krzysztof; Micklefield, Jason

Organic & Biomolecular Chemistry. 2010;8(4):782-787.

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Abstract

The immobilisation of proteins on to nanoparticles has a number of applications ranging from biocatalysis through to cellular delivery of biopharmaceuticals. Here we describe a phosphopantetheinyl transferase (Sfp)-catalysed method for immobilising proteins bearing a small 12-mer "ybbR" tag on to nanoparticles functionalised with coenzyme A. The Sfp-catalysed immobilisation of proteins on to nanoparticles is a highly efficient, single step reaction that proceeds under mild conditions and results in a homogeneous population of proteins that are covalently and site-specifically attached to the surface of the nanoparticles. Several enzymes of interest for biocatalysis, including an arylmalonate decarboxylase (AMDase) and a glutamate racemase (GluR), were immobilised on to nanoparticles using this approach. These enzymes retained their activity and showed high operational stability upon immobilisation.

Bibliographic metadata

Content type:
Publication type:
Publication form:
Published date:
Language:
eng
Abbreviated journal title:
ISSN:
Place of publication:
England
Volume:
8
Issue:
4
Start page:
782
End page:
787
Pagination:
782-787
Digital Object Identifier:
10.1039/b916773k
Pubmed Identifier:
20135034
Access state:
Active

Institutional metadata

University researcher(s):

Record metadata

Manchester eScholar ID:
uk-ac-man-scw:77988
Created by:
Micklefield, Jason
Created:
22nd March, 2010, 11:45:05
Last modified by:
Micklefield, Jason
Last modified:
23rd March, 2010, 20:38:42

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