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Tissue section AFM: In situ ultrastructural imaging of native biomolecules.

Graham, Helen K; Hodson, Nigel W; Hoyland, Judith A; Millward-Sadler, Sarah J; Garrod, David; Scothern, Anthea; Griffiths, Christopher E M; Watson, Rachel E B; Cox, Thomas R; Erler, Janine T; Trafford, Andrew W; Sherratt, Michael J

Matrix Biol. 2010;29(4):254-60.

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Abstract

Conventional approaches for ultrastructural high-resolution imaging of biological specimens induce profound changes in bio-molecular structures. By combining tissue cryo-sectioning with non-destructive atomic force microscopy (AFM) imaging we have developed a methodology that may be applied by the non-specialist to both preserve and visualize bio-molecular structures (in particular extracellular matrix assemblies) in situ. This tissue section AFM technique is capable of: i) resolving nm-microm scale features of intra- and extracellular structures in tissue cryo-sections; ii) imaging the same tissue region before and after experimental interventions; iii) combining ultrastructural imaging with complimentary microscopical and micromechanical methods. Here, we employ this technique to: i) visualize the macro-molecular structures of unstained and unfixed fibrillar collagens (in skin, cartilage and intervertebral disc), elastic fibres (in aorta and lung), desmosomes (in nasal epithelium) and mitochondria (in heart); ii) quantify the ultrastructural effects of sequential collagenase digestion on a single elastic fibre; iii) correlate optical (auto fluorescent) with ultrastructural (AFM) images of aortic elastic lamellae.

Bibliographic metadata

Type of resource:
Content type:
Published date:
Abbreviated journal title:
ISSN:
Place of publication:
Netherlands
Volume:
29
Issue:
4
Pagination:
254-60
Digital Object Identifier:
10.1016/j.matbio.2010.01.008
Pubmed Identifier:
20144712
Pii Identifier:
S0945-053X(10)00028-4
Access state:
Active

Institutional metadata

University researcher(s):

Record metadata

Manchester eScholar ID:
uk-ac-man-scw:81255
Created by:
Griffiths, Christopher
Created:
18th May, 2010, 10:22:06
Last modified by:
Griffiths, Christopher
Last modified:
1st March, 2014, 12:53:03

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