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Ribosome-associated nucleophosmin 1: increased expression and shuttling activity distinguishes prognostic subtypes in chronic lymphocytic leukaemia.

Rees-Unwin, Karen S; Faragher, Robin; Unwin, Richard D; Adams, Julie; Brown, Philip J; Buckle, Ann-Marie; Pettitt, Andrew; Hutchinson, Claire V; Johnson, Suzanne M; Pulford, Karen; Banham, Alison H; Whetton, Anthony D; Lucas, Guy; Mason, David Y; Burthem, John

Br J Haematol. 2010;148(4):534-43.

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Abstract

Two distinct groups of chronic lymphocytic leukaemia (CLL) are distinguished by the presence or absence of somatic hypermutation of the immunoglobulin heavy-chain gene. CLL without somatic hypermutation has an adverse outcome, but the precise biological differences that underlie this more aggressive clinical-course are unclear. Using a proteomic approach, we found that the two prognostic forms of CLL were consistently distinguished according to their protein expression pattern. The most important difference observed related to the different expression of nucleophosmin 1 between the two forms of CLL. This different expression was not related to apoptosis, proliferation or gene mutation. However, co-immunoprecipitation experiments identified an association between nucleophosmin 1 and ribosomal proteins. Using immunocytofluorescence, nucleophosmin 1 expression was identified in the nucleoli and nucleoplasm of all cells, but in a proportion of cells, nucleophosmin had been transferred from the nucleoplasm to the cytoplasm. Both the fluorescent intensity, and the frequency of cytoplasmic nucleophosmin 1 expression, was higher in CLL without somatic hypermutation. We propose therefore, that nucleophosmin 1, in association with ribosomal proteins, undergoes nucleo-cytoplasmic shuttling in CLL. This process is most prominent in un-mutated CLL and may signify altered protein biosynthesis.

Bibliographic metadata

Type of resource:
Content type:
Published date:
Abbreviated journal title:
ISSN:
Place of publication:
England
Volume:
148
Issue:
4
Pagination:
534-43
Digital Object Identifier:
10.1111/j.1365-2141.2009.07979.x
Pubmed Identifier:
19961478
Pii Identifier:
BJH7979
Funder acknowledgement:
Access state:
Active

Institutional metadata

University researcher(s):

Record metadata

Manchester eScholar ID:
uk-ac-man-scw:81515
Created by:
Whetton, Anthony
Created:
19th May, 2010, 11:27:57
Last modified by:
Whetton, Anthony
Last modified:
1st February, 2015, 19:05:28

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