In April 2016 Manchester eScholar was replaced by the University of Manchester’s new Research Information Management System, Pure. In the autumn the University’s research outputs will be available to search and browse via a new Research Portal. Until then the University’s full publication record can be accessed via a temporary portal and the old eScholar content is available to search and browse via this archive.

Clinically failed eggs as a source of normal human embryo stem cells

De Sousa, PA; Gardner, J; Sneddon, S; Pells, S; Tye, BJ; Dand, P; Collins, DM; Stewart, K; Shaw, L; Przyborski, S; Cooke, M; McLaughlin, KJ; Kimber, SJ; Lieberman, BA; Wilmut, I; Brison, DR

Stem Cell Research. 2009;.

Access to files

Full-text and supplementary files are not available from Manchester eScholar. Full-text is available externally using the following links:

Full-text held externally

Abstract

The promise of human embryo stem cells (hESCs) for regenerative medicine is offset by the ethical and practical challenges involved in sourcing eggs and embryos for this objective. In this study we sought to isolate an hESC line from clinically failed eggs, the usage of which would not conflict with donor interests to conceive. A total of 8 blastocysts were allocated for hESC derivation from a pool of 579 eggs whose fertilization had been clinically assessed to have occurred abnormally (i.e., three pronuclei) or failed (i.e., no pronuclei) following in vitro insemination or intracytoplasmic sperm injection (ICSI). The latter were subjected to a recovery intervention consisting of either reinsemination by ICSI or parthenogenetic stimulation. One hESC line (RCM1) was obtained from a failed-to-fertilize inseminated egg recovered by parthenogenetic activation. Standard in vitro and in vivo characterization revealed this line to possess all of the properties attributed to a normal euploid hESC line. Whole-genome single-nucleotide polymorphism analysis further revealed that the line was biparental, indicating that sperm penetration had occurred, although parthenogenetic stimulation was required for activation. Our results demonstrate the viability of an alternative strategy to generate normal hESC lines from clinically failed eggs, thereby further minimizing the potential to conflict with donor reproductive interest to conceive.

Bibliographic metadata

Type of resource:
Content type:
Published date:
Journal title:
Abbreviated journal title:
ISSN:
Digital Object Identifier:
10.1016/j.scr.2009.01.002
Pubmed Identifier:
19393594
Pii Identifier:
S1873-5061(09)00002-6
Access state:
Active

Institutional metadata

University researcher(s):

Record metadata

Manchester eScholar ID:
uk-ac-man-scw:91775
Created by:
Brison, Daniel
Created:
1st October, 2010, 10:57:47
Last modified by:
Kaar, Tracy
Last modified:
28th November, 2014, 21:23:24

Can we help?

The library chat service will be available from 11am-3pm Monday to Friday (excluding Bank Holidays). You can also email your enquiry to us.