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MSci Genetics

Year of entry: 2020

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Course unit details:
Genetics RSM

Unit code BIOL20332
Credit rating 10
Unit level Level 2
Teaching period(s) Semester 2
Offered by School of Biological Sciences
Available as a free choice unit? No

Overview

This Research Skills Module is designed to develop your experimental design, report writing and practical skills. You will be introduced to genetics research techniques during four laboratory-based projects. You will use various model organisms such as plants and fruit fly and learn different techniques such as DNA sequence analysis and immunohistochemistry.

Pre/co-requisites

Unit title Unit code Requirement type Description
Molecular Biology BIOL10221 Pre-Requisite Compulsory
Genes, Evolution and Development BIOL10521 Pre-Requisite Compulsory
Principles of Developmental Biology BIOL21172 Co-Requisite Recommended
Organismal Genetics BIOL21371 Co-Requisite Compulsory
BIOL20332 Pre & Co-requisites are BIOL10521, BIOL10221 & BIOL21371

This unit is compulsory for Genetics honours students and may be selected by Biology or Biomedical Science students.

Aims

To build on students’ previous practical experience and to introduce new concepts. To provide training in the design of experiments, the analysis and interpretation of data, the presentation of results and the maintenance of a professional lab book. To help students develop relevant practical skills and provide experimental contexts that illustrate some of the theoretical models and concepts that will be taught in BIOL21371 (Organismal Genetics) and BIOL21172 (Principles of Developmental Biology).

Learning outcomes

Students will develop skills in experimental design, time management within the laboratory, team working, the analysis and interpretation of data, and the presentation of data. They will also have a working knowledge of several commonly used Genetics techniques. Students will appreciate the need for control experiments and for careful experimental manipulation in order to obtain reliable results.

Syllabus

Module 1. Dr. Andreas Prokop

You will be introduced to principles of using the fruit fly Drosophila as a powerful and widely employed genetic model organism to unravel fundamental mechanisms of development, which then often apply to higher animals and humans. In particular, you will learn how genes contributing to developmental processes can be uncovered through large scale mutational screens in the fly, and how Drosophila can then be used to investigate and understand the functions of these genes in great detail. Key techniques will comprise immunohistochemistry and image analysis, coupled with applied classical genetics as a key strategy for Drosophila research. As further transferable skill training for future project work, you will learn to compose a figure and figure legend using your own data. 

Module 2. Dr Minsung Kim and Dr Patrick Gallois

Genetic and developmental analyses of flowers.  During this module, students will learn the genetic basis of developmental patterning for diverse flower forms in species including the plant model species Arabidopsis and the sunflower family.  Techniques include: flower dissecting and anatomical analyses, gene expression analysis in mutants using GUS histochemical staining, mathematical and modelling tools to dissect the Fibonacci pattering in flowers.

Module 3. Dr Chris Knight and Dr Rok Krašovec

Genetics of density-associated mutation-rate plasticity (DAMP). During this module students will search for genes involved in bacterial DAMP. Rate of mutation to antibiotic resistance will be estimated with a fluctuation test in an organism with one particular gene knocked out. Techniques will include various microbiological procedures needed for carrying out the fluctuation test and a fluctuation analysis.

Module 4. Dr Kathy Hentges

Genetic linkage and mutation detection in human disease. During this module, students will identify a human disease gene based on linkage analysis of affected families. Upon identification, the gene will then be sequenced to determine the nature of the mutation. Techniques will include: pedigree and genotype analysis; PCR and analysis by agarose gel electrophoresis; bi-directional fluorescent DNA sequencing and DNA sequence analysis, bioinformatics methods for mutation detection and functional profiling.

Employability skills

Analytical skills
You will develop skills in experimental design to plan experiments and work together to tackle problems. Design will be modified based on the results to try to achieve desired outcomes.
Group/team working
You will work in teams of 2 or 3 to perform experiments. Teamwork will be required to work to a deadline.
Innovation/creativity
You will develop skills in experimental design to plan experiments and work together to tackle problems. Design will be modified based on the results to try to achieve desired outcomes.
Leadership
You will develop skills in experimental design to plan experiments and work together to tackle problems.
Project management
You will develop skills in experimental design to plan experiments and work together to tackle problems.
Problem solving
You will develop skills in experimental design to plan experiments and work together to tackle problems.
Research
You will develop skills in experimental design to plan experiments and work together to tackle problems.
Written communication
You will collate data and describe in powerpoint. You will use statistical analysis to describe data succinctly.

Assessment methods

Method Weight
Other 75%
Oral assessment/presentation 25%

Written assessments:

  • Design a figure based on your data and write a figure legend for module 1, 2 and 3 (50% of the unit mark)
  • Exercise to do during the practical for module 1 (5% of unit mark)
  • Maintenance of a lab book and write a lab report for module 4 (17% of unit mark)

Online assessments:

  • On-line assessments in the form of ePBL quiz (module 2, 3 and 4) 23% of unit mark)

Oral assessment

  • In class presentation (5% of unit mark)

The RSM assessment will be split 25% for each module. In each module assessment will consist of a combination of coursework and individual and group exercises taken during class. Current coursework consists of a figure with accompanying legend for module 1,2 and 3 with additional short answer questions and ePBL assessments. For module 4, the assessment will comprise a short in-class presentation, a lab report and ePBL.

RSM Attendance guidelines

Students are expected to attend all scheduled RSM sessions on time (N.B. Health and safety information will be delivered at the start of practical sessions, and students who are not present at the start may be asked to leave the lab). Students who arrive late will be marked as absent for that session.

Failure to attend a session (an unauthorised absence) will result in a 10% (i.e. 10 mark) penalty being applied to the overall RSM mark (i.e. a student obtaining a mark of 65% overall will instead receive a mark of 55%). Furthermore, any students who miss a practical session will not receive a mark for any associated post-lab assessment (N.B. this mark will be removed before calculating the average post-lab mark to avoid a student being penalised twice).

Further absences will result in further penalties (i.e. 2 absences = a penalty of 20% (as described above)).

Feedback methods

During the practical sessions, there will be many opportunities for you to get feedback from staff or demonstrators on your technical performance. The short answer questions or exercises in the practical manual are there to test your understanding and you should get feedback from staff or demonstrators on your answers. You will get feedback on your overall performance in the form of the final mark for the unit and you will get feedback for each of the assessments you have completed

Recommended reading

Either Genes VIII (paperback) or Genes IX (hardcover).

Recommended Reading

  1. Fixsen W D, Solutions manual for An Introduction to Genetic Analysis (7th edition), W. H. Freeman, 2000, Recommended
  2. Griffiths A J F, Gelbart W M, Miller J H and Lewontin R C, Modern Genetic Analysis, W. H. Freeman, 1999, Recommended
  3. Griffiths A J F, Miller J H, Suzuki D T, Lewontin R C and Gelbart W M, An Introduction to Genetic Analysis (7th edition), Freeman, 2000, Recommended
  4. Ralph Greenspan, Fly Pushing : The Theory and Practice of Drosophila Genetics, Cold Spring Harbor Laboratory Press, 1997, Recommended

Study hours

Scheduled activity hours
Practical classes & workshops 72
Independent study hours
Independent study 28

Teaching staff

Staff member Role
Karel Dorey Unit coordinator

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