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Glycoprotein Labeling Using Engineered Variants of Galactose Oxidase Obtained by Directed Evolution
Rannes, Julie B; Ioannou, Avgousta; Willies, Simon C; Grogan, Gideon; Behrens, Carsten; Flitsch, Sabine L; Turner, Nicholas J
Journal of the American Chemical Society. 2011;133(22):8436-8439.
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Abstract
A directed evolution approach has been used for the generation of variants of galactose oxidase (GOase) that can selectively oxidize glycans on glycoproteins. The aldehyde function introduced on the glycans D-mannose (Man) and D-N-acetyl glucosamine (GlcNAc) by the enzyme variants could then be used to label the glycoproteins and also whole cells that display mannosides on their surface.
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