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Fluorescent Probes of Conformational Signal Relay in Membrane Environments

Lister, Francis George Alexander

[Thesis]. Manchester, UK: The University of Manchester; 2015.

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Abstract

G-Protein Coupled Receptors (GPCRs) are a class of membrane-bound receptor proteins capable of relaying a biological signal across a cell membrane through a solely conformational change in their transmembrane domain. Previous work has shown that helical foldamers composed of achiral monomeric units can be used in an analogous manner to relay stereochemical information on the nano-scale through the conformational control of screw-sense preference. While this work has produced some highly successful examples of signal relay, mimicking the function of GPCRs, its reliance on screw-sense responsive NMR probes has restricted further development into membrane environments.This thesis describes the successful development of a pyrene based screw-sense responsive fluorescence probe and its subsequent use in the development of a series of membrane-based GPCR mimics. This thesis has also details the preliminary steps towards the development of light-responsive controllers of screw-sense preference for nano-scale signal relay devices.

Bibliographic metadata

Type of resource:
Content type:
Administered thesis
Form of thesis:
Traditional
Type of submission:
Doctoral level ETD - final
Thesis title:
Fluorescent Probes of Conformational Signal Relay in Membrane Environments
Degree type:
Doctor of Philosophy
Degree programme:
PhD Chemistry (42 month)
Publication date:
2015-12-15T10:13:00
Institution:
The University of Manchester
Location:
Manchester, UK
Total pages:
223
Abstract:
G-Protein Coupled Receptors (GPCRs) are a class of membrane-bound receptor proteins capable of relaying a biological signal across a cell membrane through a solely conformational change in their transmembrane domain. Previous work has shown that helical foldamers composed of achiral monomeric units can be used in an analogous manner to relay stereochemical information on the nano-scale through the conformational control of screw-sense preference. While this work has produced some highly successful examples of signal relay, mimicking the function of GPCRs, its reliance on screw-sense responsive NMR probes has restricted further development into membrane environments.This thesis describes the successful development of a pyrene based screw-sense responsive fluorescence probe and its subsequent use in the development of a series of membrane-based GPCR mimics. This thesis has also details the preliminary steps towards the development of light-responsive controllers of screw-sense preference for nano-scale signal relay devices.
Keyword(s):
Thesis main supervisor(s):
Degree grantor:
Language:
en

Record metadata

Manchester eScholar ID:
uk-ac-man-scw:288851
Created by:
Lister, Francis
Created:
15th December, 2015, 10:13:01
Last modified by:
Lister, Francis
Last modified:
9th September, 2016, 13:01:16

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